SpermComet - Lab Information

Surgically retrieved sperm protocol

1) Please label cryovials (two per patient) with patient forename, surname, DOB and

your clinic ID

2) All surgically retrieved sperm should be mixed with the cryoprotectant

SpermFreeze™.

3) Add 0.7ml of SpermFreeze™ to 1ml of surgically retrieved sperm. Do so by added

SpermFreeze™ drop by drop, being carefully to ensure there are no air bubbles.

4) Allow cryoprotectant and sample to equilibrate at room temperature for 10mins

5) Plunge cryovials into liquid nitrogen to snap freeze and store at -196°C until

transport to LFT

6) Once samples have been added to the shipper, please ensure that the foam cork/lid

is securely placed on the shipper for return to LFT. Failure to do so will result in

temperature failure and loss of the samples.

7) Once samples are loaded, close mushroom container and fasten shut. Place

container at agreed pickup point and the LFT couriers will collect at the agreed time.